D. MOLECULAR GENETICS

The requirement of the Molecular Genetics Course must reach 3 credits.  

This course is designed to provide students with a background in molecular genetics. The topics covered include an examination of the structure and replication of DNA, the molecular mechanisms underlying the recombination of DNA, the molecular basis of gene regulation and how gene expression is tied to intracellular and extracellular factors by signal transduction pathways

 

Course

Title

Credits

Course D.1 Introduction to Molecular Genetics:
  • The role of genes within cells
  • Principles of recombinant DNA technology
1 Credit
Course D.2 PCR and Reverse Genetics:
  • Polymerase chain reaction 
  • PCR applications 
  • In vitro mutagenesis
1 Credit
Course D.3 Gene Expression
  • Transcription: 
  • RNA polymerases 
  • Structure of promoter 
  • Regulation of transcription

  • RNA processing and degradation:
  • RNA capping and polyadenylation 
  • RNA splicing and transport
  • RNA stability and degradation 

  • Genetic code and translation: 
  • Translational machinery 
  • Genetic code and codon usage
  • Initiation of translation
  • Elongation and termination of translation 

  • Protein processing, folding and degradation: 
  • Protein processing and splicing 
  • Protein stability and degradation 
  • Regulatory functions of protein phosphorylation 
  • Protein folding and chaperones 
  • Prions and protein-based inheritance 

2 Credits
Course D.4 Genome Structure and Stability
  • Genome structure and replication: 
  • Chromatin organization
  • Chromosome replication and segregation 

  • DNA repair and recombination:
  • Mechanisms of DNA repair
  • Mechanisms of homologous recombination 
  • Programmed DNA rearrangements
  • Molecular mechanisms of immune recognition 

  • Transposable elements:
  • DNA transposons 
  • Retrotransposons and retroviruses 
  • Mechanisms of reverse transcription
2 Credits
Course D.5 Genetic Engineering of Eukaryotic Organisms.
  • Yeast genetic engineering:
  • Organization of the yeast cell and genome 
  • Yeast plasmids and vectors 
  • Yeast contribution to molecular genetics 

  • Animal and plant genetic engineering:
  • Genetic engineering of animal cells in the culture 
  • Transgenic animals 

  • Studying whole genomes: genomic sequencing
    and genome projects
2 Credits
Course D.6 Molecular Bases of Evolution:
  • Evolution of the translational machinery 
  • Origin of introns 
  • Evolution of the genome 
  • Evolution of the cell and genetic systems
    of eukaryotic organelles 
  • Molecular evidences of human evolution. 
2 Credits